diff --git a/CHANGELOG.md b/CHANGELOG.md
index 378335207..f655b0888 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -7,6 +7,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ## [Unreleased]
 
+### Fixed
+
+- Don’t add spaces to negative numbers following a comma ([#12324](https://github.com/tailwindlabs/tailwindcss/pull/12324))
+
 ## [3.3.5] - 2023-10-25
 
 ### Fixed
diff --git a/src/util/dataTypes.js b/src/util/dataTypes.js
index 005c37ad1..06ccb9524 100644
--- a/src/util/dataTypes.js
+++ b/src/util/dataTypes.js
@@ -77,7 +77,7 @@ export function normalize(value, context = null, isRoot = true) {
 
 /**
  * Add spaces around operators inside math functions
- * like calc() that do not follow an operator or '('.
+ * like calc() that do not follow an operator, '(', or `,`.
  *
  * @param {string} value
  * @returns {string}
@@ -164,7 +164,7 @@ function normalizeMathOperatorSpacing(value) {
       // Handle operators
       else if (
         ['+', '-', '*', '/'].includes(char) &&
-        !['(', '+', '-', '*', '/'].includes(lastChar())
+        !['(', '+', '-', '*', '/', ','].includes(lastChar())
       ) {
         result += ` ${char} `
       } else {
diff --git a/tests/normalize-data-types.test.js b/tests/normalize-data-types.test.js
index f11e38b79..b249f7874 100644
--- a/tests/normalize-data-types.test.js
+++ b/tests/normalize-data-types.test.js
@@ -69,6 +69,9 @@ let table = [
   ['calc(theme(spacing.foo-2))', 'calc(theme(spacing.foo-2))'],
   ['calc(theme(spacing.foo-bar))', 'calc(theme(spacing.foo-bar))'],
 
+  // A negative number immediately after a `,` should not have spaces inserted
+  ['clamp(-3px+4px,-3px+4px,-3px+4px)', 'clamp(-3px + 4px,-3px + 4px,-3px + 4px)'],
+
   // Prevent formatting inside `var()` functions
   ['calc(var(--foo-bar-bar)*2)', 'calc(var(--foo-bar-bar) * 2)'],